Journal: Neuron

Article Title: Sex-specific role for the long non-coding RNA LINC00473 in depression

doi: 10.1016/j.neuron.2020.03.023

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: See for primer sequences. table ft1 table-wrap mode="anchored" t5 caption a7 REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Clozapine N-oxide Enzo Life Sciences Cat. #: BML-NS105 Lipofectamine RNAiMAX Thermo Fisher Cat. #: 13778150 Critical Commercial Assays RNeasy Micro Kits Qiagen Cat. #: 74004 iScript BioRad Cat. #: 1708891 Fast SYBRTM Green Master Mix Applied Biosystems Cat. #: 4334973 ScriptSeq Complete Kit Epicentre Cat. #: BHMR1224 Corticosterone Assay R&D Systems Cat. #: KGE009 RNAscope Multiplex Fluorescent reagent kit version 2 Advanced Cell Diagnostics Cat. #: 323110 Fluorescein plus evaluation kit Perkin Elmer Cat. #: 741E001KT NEBNext Ultra DNA Library Prep kit New England Biolabs Cat. #: E7645S/L Deposited Data RNA-Seq Human depression postmortem ( Labonté et al., 2017 ) N/A RNA-Seq Human depression postmortem ( Zhou et al., 2018 ) N/A RNA-seq LINC00473 KD in NPCs This Study {"type":"entrez-geo","attrs":{"text":"GSE138677","term_id":"138677"}} GSE138677 RNA-seq LINC00473 expression in mouse This Study {"type":"entrez-geo","attrs":{"text":"GSE138677","term_id":"138677"}} GSE138677 ChIRP seq LINC00473 SHSY-5Y cells This Study {"type":"entrez-geo","attrs":{"text":"GSE138677","term_id":"138677"}} GSE138677 Experimental Models: Cell Lines Neuron Progenitor Cells (NPCs) ( Hoffman et al., 2017 ) N/A SH-SY5Y ATCC ATCC CRL-2266 Experimental Models: Organisms/Strains C57Bl6/j Jackson Laboratory Cat. #: 000664 Oligonucleotides sequences listed in Supplementary Table S3 HS-LINC00473 probes (RNAScope) Advanced Cell Diagnostics Cat. #: 464821 Recombinant DNA p1005-LINC00473-eGFP This study N/A p1005-eGFP This study N/A rAAV2/hSyn-DIO-hM3D(Gq)-mCherry ( Krashes et al., 2011 ) Addgene, Cat. #: 44361-AAV2 Software and Algorithms Ethovision XT Noldus https://www.noldus.com/ethovision-xt Fiji/ImageJ NIH https://imagej.net/Fiji/Downloads Prism 8 GraphPad https://www.graphpad.com/scientific-software/prism/ R GNU package https://www.r-project.org/ QuantStudio 7 Thermo Fisher https://www.thermofisher.com/us/en/home/global/forms/life-science/quantstudio-6-7-flex-software.html Open in a separate window KEY RESOURCES TABLE

Techniques: Recombinant, SYBR Green Assay, RNAscope, Multiplex Assay, Expressing, Software